The fixture will mark England's first visit to the Spanish capital since their controversial meeting at the Santiago Bernabeu in 2004, when several Black England players were subjected to racist abuse from sections of the home crowd.
The issue has resurfaced following another incident involving Spanish supporters last month. The Spanish Football Federation was fined and ordered to partially close the stadium for its next friendly after fans directed Islamophobic and xenophobic chants towards Muslims during a match against Egypt.
Speaking ahead of Sunday's encounter, Tuchel confirmed that he would discuss Uefa's procedures with his squad to ensure everyone understands how to respond if discriminatory behaviour occurs during the match.
The German manager explained that a similar conversation had taken place before England's match against Serbia in Belgrade in September 2025. He added that the players would be reminded of the measures available should an incident arise in Madrid.
Uefa has established a three-stage protocol to address discrimination inside stadiums, giving referees the authority to suspend matches and abandon them if unacceptable behaviour continues.
The first step involves stopping the match and requesting a public announcement warning spectators against discriminatory conduct. If the abuse persists, the referee can temporarily suspend proceedings for five to 10 minutes and instruct both teams to leave the pitch.
Should the behaviour continue after the second intervention, officials have the authority to abandon the match entirely.
Tuchel stressed the importance of ensuring his players understand these procedures, adding that their strongest response to any potential hostility would be their performance on the pitch.
England will face Spain at Atletico Madrid's Metropolitano Stadium, with victory guaranteeing them top spot in their Nations League group and a more favourable quarter-final draw.
However, the challenge will be considerable, with Spain currently unbeaten in 43 matches. The reigning world champions also defeated England 3-2 at Wembley in September.
Tuchel believes his team must approach.
